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Addgene

5aa SunTag VP64 CLV3
(Plasmid #115485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMOA34
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
  • Alt name
    5aa SunTag VP64 CLV3
  • Insert Size (bp)
    15407

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Portions of this construct were amplified from Addgene plasmid #60903 and #60904, gifts from Dr. Ron Vale. The pMOA34 backbone was provided by Dr. Zachary Nimchuk.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5aa SunTag VP64 CLV3 was a gift from Steven Jacobsen (Addgene plasmid # 115485 ; http://n2t.net/addgene:115485 ; RRID:Addgene_115485)

  • For your References section:

    Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722
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