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Addgene

RCAS-myc
(Plasmid #11548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11548 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCAS
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    c-myc
  • Alt name
    myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NT_008046
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pme1 (destroyed during cloning)
  • 3′ cloning site Pme1 (destroyed during cloning)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The RCAS vectors that carry oncogenes are harmless for mammalian cells, but can be oncogenic in avian cells since some avian
cells express the receptor for the RCAS virus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS-myc was a gift from Sandra Orsulic (Addgene plasmid # 11548 ; http://n2t.net/addgene:11548 ; RRID:Addgene_11548)

  • For your References section:

    Induction of ovarian cancer by defined multiple genetic changes in a mouse model system. Orsulic S, Li Y, Soslow RA, Vitale-Cross LA, Gutkind JS, Varmus HE. Cancer Cell. 2002 Feb . 1(1):53-62. 10.1016/S1535-6108(01)00002-2 PubMed 12086888
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