pGPA1
(Plasmid #115476)

• Purpose: (Empty Backbone) Generation of mariner-based transposon mutant libraries in Enterococcus faecium
• Depositing Lab: Willem van Schaik
• Publication: Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi: 10.1186/s12864-017-4299-9.

Backbone

• Vector backbone: pZXL5
• Backbone size (bp): 11760
• Modifications to backbone: Made two point mutations in the inverted terminal repeats of the himar1 transposon, introducing two MmeI sites
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Gentamicin, 25 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Both chloramphenicol and gentamicin can be used as selective agents. Replicates in E. coli at 37°C, but is temperature sensitive in E. faecium (replicates at 30°C, but not at 37°C).
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry