RCAS-myrAkt
(Plasmid #11547)

• Depositing Lab: Sandra Orsulic
• Publication: Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Backbone

• Vector backbone: RCAS
• Backbone size w/o insert (bp): 11600
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Protein Kinase B
• Alt name: Akt1
• Alt name: PKB
• Alt name: PKBalpha
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• GenBank ID: NC_000078
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Tags / Fusion Proteins:
  • myr signal (N terminal on insert)
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Pme1 (destroyed during cloning)
• 3′ cloning site: Pme1 (destroyed during cloning)
• 5′ sequencing primer: RCAS-F

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The RCAS vectors that carry oncogenes are harmless for mammalian cells, but can be oncogenic in avian cells since some avian
cells express the receptor for the RCAS virus.