pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
(Plasmid #115432)

• Purpose: Vector for expressing RPL28 with mutagenized exon2
• Depositing Lab: Nicholas Ingolia
• Publication: McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856. doi: 10.1021/acssynbio.8b00529. Epub 2019 Apr 2.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 2500
• Total vector size (bp): 5527
• Modifications to backbone: Yeast ARS/CEN
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RPL28
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1023
• Mutation: exon 2 deleted, with MfeI / NheI cloning site
• GenBank ID: NM_001180968
• Entrez Gene: RPL28 (a.k.a. YGL103W, CYH2)
• Promoter: endogenous

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: HIS5
• Species: S. pombe (fission yeast); Candida albicans
• Insert Size (bp): 1425
• Promoter: Ashbya gospii TEF1

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pNTI619 HIS RPL28 MfeNhe vector.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry