pPGK-Cre-bpA
(Plasmid #11543)

• Depositing Lab: Klaus Rajewsky
• Publication: Rajewsky pPGK-Cre-bpA (unpublished)

Backbone

• Vector backbone: XhopKS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2906
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Phosphoglycerate kinase 1 promoter
• Alt name: PGK
• Species: bacteriophage P1
• Insert Size (bp): 1944

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sall (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full Gene Name: PGK-Cre-bpA: Phosphoglycerate kinase I promoter (PGK), the bacteriophage P1 recombinase Cre with a SV40 large T antigen nuclear localization signal (NLS-Cre), bovine growth hormone polyadenylation site (bpA). Use to express the Cre recombinase in mammalian cells. The vector backbone is a variant of pBluescriptKS- (Stratagene), in which the Xbal site was converted to an Xhol site (provided by Paul Hasty) (Soriano P., Cell 1997). The plasmid was generated by Kurt Fellenberg. He cloned a NLS-Cre sequence, as published by Hua Gu (Gu H, Zou YR, Rajewsky K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164) and cloned it into the pPGK-neo-bpA (Soriano P, Montgomery C, Geske R, Bradley A. 1991 Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell. Feb 22;64(4):693-702.), replacing the neomycin resistance gene (neo) with the NLS-Cre.

NOTE: There are some discrepancies between the Addgene quality control sequence and the sequence provided by the depositing lab, but these differences should not affect plasmid function.