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Addgene

pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)
(Plasmid #115429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size (bp) 2500
  • Modifications to backbone
    Yeast ARS/CEN
  • Vector type
    Yeast Expression
  • Promoter PGK1
  • Selectable markers
    HIS3
  • Tags / Fusion Proteins
    • λN (C terminal on insert)
    • T2A-SpHIS5 (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347) was a gift from Nicholas Ingolia (Addgene plasmid # 115429 ; http://n2t.net/addgene:115429 ; RRID:Addgene_115429)

  • For your References section:

    An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries. McGeachy AM, Meacham ZA, Ingolia NT. ACS Synth Biol. 2019 Apr 19;8(4):844-856. doi: 10.1021/acssynbio.8b00529. Epub 2019 Apr 2. 10.1021/acssynbio.8b00529 PubMed 30908907
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