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Addgene

104b-U1(A503V/A509D)
(Plasmid #1154)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4895
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSP104
  • Alt name
    5929
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3545
  • Mutation
    A503V/A509D
  • GenBank ID
    M67479
  • Entrez Gene
    HSP104 (a.k.a. YLL026W)
  • Tag / Fusion Protein
    • GAL1:10 promoter (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer GAAGCCGCCGAGCGGGTGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    104b-U1(A503V/A509D) was a gift from Susan Lindquist (Addgene plasmid # 1154 ; http://n2t.net/addgene:1154 ; RRID:Addgene_1154)

  • For your References section:

    Dominant gain-of-function mutations in Hsp104p reveal crucial roles for the middle region. Schirmer EC, Homann OR, Kowal AS, Lindquist S. Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20. 10.1091/mbc.e02-08-0502 PubMed 14978213
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