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Addgene

AAEL004399 GPB5
(Plasmid #115395)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115395 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME18s
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAEL004399 GPB5
  • Species
    Aedes aegypti
  • Entrez Gene
    LOC5564685 (a.k.a. AaeL_AAEL004399, AAEL004399, GPRFSH, LGR1)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.

Note: Plasmid contains amino acids 235-941 of GPA2/GPB5 glycoprotein hormone receptor. The N-terminal 55 amino acids do not BLAST to any known sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAEL004399 GPB5 was a gift from Leslie Vosshall (Addgene plasmid # 115395 ; http://n2t.net/addgene:115395 ; RRID:Addgene_115395)

  • For your References section:

    Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting. Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB. Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004. 10.1016/j.cell.2018.12.004 PubMed 30735632
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