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Addgene

AAEL011325 AKH R
(Plasmid #115389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME18s
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAEL011325 AKH R
  • Species
    Aedes aegypti
  • Entrez Gene
    LOC5574685 (a.k.a. AaeL_AAEL011325, AAEL011325, AKHR-1, AKHR-2)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.

Note: Plasmid contains a D11G mutation in AAEL011325 and a 53bp intron not present in the depositor sequence. D11G is present in NCBI reference sequence XP_021700044.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAEL011325 AKH R was a gift from Leslie Vosshall (Addgene plasmid # 115389 ; http://n2t.net/addgene:115389 ; RRID:Addgene_115389)

  • For your References section:

    Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting. Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB. Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004. 10.1016/j.cell.2018.12.004 PubMed 30735632
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