AAEL006283 MS R
(Plasmid
#115386)
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PurposeExpresses Ae. aegypti receptor in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 115386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepME18s
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAEL006283 MS R
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SpeciesAedes aegypti
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Entrez GeneLOC5567691 (a.k.a. AaeL_AAEL006283, AAEL006283, GPRDMS)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAEL006283 MS R was a gift from Leslie Vosshall (Addgene plasmid # 115386 ; http://n2t.net/addgene:115386 ; RRID:Addgene_115386) -
For your References section:
Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting. Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB. Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004. 10.1016/j.cell.2018.12.004 PubMed 30735632