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Addgene

AAEL012796
(Plasmid #115383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME18s
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAEL012796
  • Species
    Aedes aegypti
  • Entrez Gene
    LOC5576812 (a.k.a. AaeL_AAEL012796, AAEL012796, GPRGHP3)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.

Note: Plasmid contains the first 433 of 682 amino acids of pyrokinin-1, and also contains a F166L mutation in pyrokinin-1 compared to NCBI reference sequence XP_021694461.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAEL012796 was a gift from Leslie Vosshall (Addgene plasmid # 115383 ; http://n2t.net/addgene:115383 ; RRID:Addgene_115383)

  • For your References section:

    Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting. Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB. Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004. 10.1016/j.cell.2018.12.004 PubMed 30735632
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