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pTH825-CEN-minRLuc/slowstaCFLuc
(Plasmid #115370)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115370 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTH644
  • Backbone size w/o insert (bp) 6615
  • Total vector size (bp) 9692
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Gcn4 5'-UTR + Firefly Luciferase
  • Alt name
    CFLuc
  • Species
    Synthetic; Photinus pyralis
  • Insert Size (bp)
    2149
  • Mutation
    The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes.
  • GenBank ID
    AF043450
  • Promoter TDH3

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GTATATAAAGAACGGTAGG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Renilla luciferase (codon minimised)
  • Alt name
    RLuc
  • Species
    Synthetic; Renilla reniformis
  • Insert Size (bp)
    942
  • Mutation
    All codons have been changed for the least favourable codons for yeast expression
  • GenBank ID
    AF043450.1
  • Promoter ADH1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CCTGCAATTATTAATCTTTTGTTTCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH825-CEN-minRLuc/slowstaCFLuc was a gift from Tobias von der Haar (Addgene plasmid # 115370 ; http://n2t.net/addgene:115370 ; RRID:Addgene_115370)
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