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Addgene

phRL TK 5BoxB sp 36
(Plasmid #115365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTK
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla Luciferase
  • Alt name
    5BoxB
  • Promoter TK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer use reverse primer
  • 3′ sequencing primer EBV-rev [5'-GTGGTTTGTCCAAACTCATC-3']
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

clone number 122. BoxB repeat sequence: gggccctgaagaagggccc

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phRL TK 5BoxB sp 36 was a gift from Witold Filipowicz & Ramesh Pillai (Addgene plasmid # 115365 ; http://n2t.net/addgene:115365 ; RRID:Addgene_115365)

  • For your References section:

    Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. Pillai RS, Artus CG, Filipowicz W. RNA. 2004 Oct . 10(10):1518-25. 10.1261/rna.7131604 PubMed 15337849
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