pCIneo-RL-5BoxB
(Plasmid
#115364)
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PurposeExpression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTR
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCIneo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla Luciferase
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Alt name5BoxB
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
clone number 144. BoxB repeat sequence: gggccctgaagaagggccc
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIneo-RL-5BoxB was a gift from Witold Filipowicz & Ramesh Pillai (Addgene plasmid # 115364 ; http://n2t.net/addgene:115364 ; RRID:Addgene_115364) -
For your References section:
Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. Pillai RS, Artus CG, Filipowicz W. RNA. 2004 Oct . 10(10):1518-25. 10.1261/rna.7131604 PubMed 15337849
