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pCIneo-NHA-LacZ
(Plasmid #115363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lacZ beta-D-galactosidase
  • Promoter CMV
  • Tag / Fusion Protein
    • N peptide and HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

clone number 103. N-peptide = MDAQTRRRERRAEKQAQWKAAN

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo-NHA-LacZ was a gift from Witold Filipowicz & Ramesh Pillai (Addgene plasmid # 115363 ; http://n2t.net/addgene:115363 ; RRID:Addgene_115363)

  • For your References section:

    Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. Pillai RS, Artus CG, Filipowicz W. RNA. 2004 Oct . 10(10):1518-25. 10.1261/rna.7131604 PubMed 15337849
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