pCIneo-NHA
(Plasmid #115359)

• Purpose: Cloning vector to produce NHA-fusion proteins without any other gene insert
• Depositing Labs: Witold Filipowicz, Ramesh Pillai
• Publication: Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Backbone

• Vector backbone: pCIneo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N peptide and HA
• Insert Size (bp): 160
• Promoter: CMV
• Tag / Fusion Protein:
  • N peptide and HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

clone number 212. N-peptide = MDAQTRRRERRAEKQAQWKAAN