Skip to main content
Addgene

pcDNA3.1-HDAC2-H141A
(Plasmid #115346)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7292
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone deacetylase 2
  • Alt name
    Hdac2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1864
  • Mutation
    H141A
  • GenBank ID
    NM_008229
  • Entrez Gene
    Hdac2 (a.k.a. D10Wsu179e, YAF1, Yy1bp, mRPD3)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVf
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HDAC2-H141A was a gift from Javier Gonzalez-Maeso (Addgene plasmid # 115346 ; http://n2t.net/addgene:115346 ; RRID:Addgene_115346)

  • For your References section:

    HDAC2 regulates atypical antipsychotic responses through the modulation of mGlu2 promoter activity. Kurita M, Holloway T, Garcia-Bea A, Kozlenkov A, Friedman AK, Moreno JL, Heshmati M, Golden SA, Kennedy PJ, Takahashi N, Dietz DM, Mocci G, Gabilondo AM, Hanks J, Umali A, Callado LF, Gallitano AL, Neve RL, Shen L, Buxbaum JD, Han MH, Nestler EJ, Meana JJ, Russo SJ, Gonzalez-Maeso J. Nat Neurosci. 2012 Sep;15(9):1245-54. doi: 10.1038/nn.3181. Epub 2012 Aug 5. 10.1038/nn.3181 PubMed 22864611
Related items:
Commonly requested with: