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Addgene

pEG302-SunTag-FWAgRNA-4-22aa-TET1cd
(Plasmid #115344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEG302
  • Total vector size (bp) 27478
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
  • Alt name
    SunTagFWAg4-22aa-TET1cd
  • Species
    H. sapiens (human), A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    17426

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Portions of the SunTagng-22aa-TET1cd were amplified from Addgene plasmid # 60903 and # 60904, gifts from Ron Vale.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert is the same as what appears in Plasmid #106435. The plasmid backbone was changed to increase transformation efficiency.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG302-SunTag-FWAgRNA-4-22aa-TET1cd was a gift from Steven Jacobsen (Addgene plasmid # 115344 ; http://n2t.net/addgene:115344 ; RRID:Addgene_115344)
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