Skip to main content
Addgene

pLNCX2-mCherry-VPS4A
(Plasmid #115334)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLNCX2
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VPS4A
  • Species
    H. sapiens (human)
  • Mutation
    silent mutation in position 4050 C to T
  • Entrez Gene
    VPS4A (a.k.a. CIMDAG, SKD1, SKD1A, SKD2, VPS4, VPS4-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV
  • 3′ sequencing primer mCherry-FWD
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNCX2-mCherry-VPS4A was a gift from Sanford Simon (Addgene plasmid # 115334 ; http://n2t.net/addgene:115334 ; RRID:Addgene_115334)

  • For your References section:

    Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly. Johnson DS, Bleck M, Simon SM. Elife. 2018 Jul 4;7. pii: 36221. doi: 10.7554/eLife.36221. 36221 [pii] PubMed 29972351
Related items:
Commonly requested with: