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Addgene

pBAD-TrxRFP1
(Plasmid #115327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisB
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TrxRFP1
  • Species
    Synthetic
  • Insert Size (bp)
    1300
  • Promoter pBAD
  • Tag / Fusion Protein
    • His-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD forward
  • 3′ sequencing primer pBAD reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-TrxRFP1 was a gift from Huiwang Ai (Addgene plasmid # 115327 ; http://n2t.net/addgene:115327 ; RRID:Addgene_115327)

  • For your References section:

    Monitoring thioredoxin redox with a genetically encoded red fluorescent biosensor. Fan Y, Makar M, Wang MX, Ai HW. Nat Chem Biol. 2017 Jun 26. doi: 10.1038/nchembio.2417. 10.1038/nchembio.2417 PubMed 28671680
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