NODAL-matDYK-C312S
(Plasmid
#115259)
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PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal DYK tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6
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Backbone manufacturerOrigene
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNODAL
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SpeciesH. sapiens (human)
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MutationC312S
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Entrez GeneNODAL (a.k.a. HTX5)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal DYK tag at N-terminal end of mature peptide. Internal DYK tag can be difficult to detect in full-length peptide. C312 is involved in interchain disuflide bond formation (by similarity) . Please visit https://www.biorxiv.org/content/early/2018/03/04/276170 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NODAL-matDYK-C312S was a gift from Lynne Postovit (Addgene plasmid # 115259 ; http://n2t.net/addgene:115259 ; RRID:Addgene_115259) -
For your References section:
Genetically regulated human NODAL splice variants are differentially post-transcriptionally processed and functionally distinct. Findlay SD, Bilyk O, Lypka K, Waskiewicz AJ, Postovit L. (2018) bioRxiv 276170 10.1101/276170