pAAV-UbC-mCherry Rab5
(Plasmid #115239)

• Purpose: To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
• Depositing Lab: Pantelis Tsoulfas
• Publication: Eukaryotic expression plasmids for expression and visualization of early endocytic markers Rab5 and Rab7 (unpublished)

Backbone

• Vector backbone: pAAV-SIBR-anti_I
• Backbone manufacturer: Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
• Backbone size w/o insert (bp): 5235
• Total vector size (bp): 6575
• Modifications to backbone: The anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab5a
• Alt name: NP_004153.2
• Species: H. sapiens (human); Homo sapiens
• Insert Size (bp): 1340
• GenBank ID: NP_004153.2
• Entrez Gene: RAB5A (a.k.a. RAB5)
• Promoter: Human UbC
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: pCAG_F_primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Plasmid contains a mutation that removes the first nine amino acids of mCherry. This mutation is not known to affect plasmid function.