pAAV-UbC-mEmerald Rab7a
(Plasmid #115238)

• Purpose: To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.
• Depositing Lab: Pantelis Tsoulfas
• Publication: Eukaryotic expression plasmids for expression and visualization of early endocytic markers Rab5 and Rab7 (unpublished)

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Backbone

• Vector backbone: pAAV-SIBR-anti_I
• Backbone manufacturer: Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
• Backbone size w/o insert (bp): 5235
• Total vector size (bp): 6627
• Modifications to backbone: The anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab7a fused to mEmerald
• Alt name: NM_004637.5
• Species: H. sapiens (human)
• Insert Size (bp): 1416
• Mutation: 7 Amino Acids (21 Nucleotides) between mEmerald and Rab7a
• GenBank ID: NM_004637.5
• Entrez Gene: RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
• Promoter: Human UbC
• Tags / Fusion Proteins:
  • mEmerald (N terminal on insert)
  • mEmerald (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: pCAG_F_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ADDGENE plasmid #54244

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry