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pLEX307_CR-PDHA2S293D
(Plasmid #115201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX-307
  • Backbone manufacturer
    David Root, Addgene plasmid # 41392
  • Backbone size w/o insert (bp) 8270
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CR (CRISPR/Cas9-resistant)-PDHA2S293D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1167
  • Mutation
    c.321C>T (silent when translated to protein, confers CRISPR/Cas9 resistance since it mutates the canonical PAM site), p.S293D
  • GenBank ID
    NM_005390.4, NP_005381.1
  • Entrez Gene
    PDHA2 (a.k.a. PDHAL, MGC149517, MGC149518)
  • Promoter EF1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer EF1alpha_F
  • 3′ sequencing primer WPRE_R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307_CR-PDHA2S293D was a gift from Mohan Babu (Addgene plasmid # 115201 ; http://n2t.net/addgene:115201 ; RRID:Addgene_115201)

  • For your References section:

    The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF. Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA, Golshani A, Babu M. Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585. 10.1093/nar/gkab585 PubMed 34244791
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