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pENTR223-PARK7E163K
(Plasmid #115181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR223
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 2790
  • Vector type
    gateway cloning into appropriate destination vectors

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PARK7E163K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    567
  • Mutation
    p.E163K
  • Entrez Gene
    PARK7 (a.k.a. DJ-1, DJ1, GATD2, HEL-S-67p)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13_F
  • 3′ sequencing primer M13_R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR223-PARK7E163K was a gift from Mohan Babu (Addgene plasmid # 115181 ; http://n2t.net/addgene:115181 ; RRID:Addgene_115181)

  • For your References section:

    Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y, Foster LJ, Bader GD, Cayabyab FS, Babu M. iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057. 10.1016/j.isci.2019.08.057 PubMed 31536960
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