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Addgene

pSuper-Sema4A 42-3n
(Plasmid #115173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper
  • Backbone size w/o insert (bp) 4000
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA targeting Sema4A
  • gRNA/shRNA sequence
    ACAAAGCCTTGACCTTCAT
  • Species
    M. musculus (mouse), R. norvegicus (rat)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper-Sema4A 42-3n was a gift from Suzanne Paradis (Addgene plasmid # 115173 ; http://n2t.net/addgene:115173 ; RRID:Addgene_115173)

  • For your References section:

    Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and glutamatergic synapse development in the mammalian hippocampus. McDermott JE, Goldblatt D, Paradis S. Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 10.1016/j.mcn.2018.06.008. 10.1016/j.mcn.2018.06.008 PubMed 29981480
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