pDest-566
(Plasmid #11517)

• Purpose: (Empty Backbone)
• Depositing Lab: Dominic Esposito
• Publication: Gateway Destination vectors for E. coli expression (unpublished)

Backbone

• Vector backbone: pDest-560
• Backbone manufacturer: PEL
• Backbone size (bp): 9367
• Vector type: Bacterial Expression ; Gateway Destination Vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: Must be grown in a strain resistant to ccdB (DB3.1, ccdB Survival, or F').
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • His6-MBP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MBP fragment from New England Biolabs pMAL-c2 vector
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry