pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA
(Plasmid #115161)

• Purpose: This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.
• Depositing Lab: William Wisden
• Publication: Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.e4. doi: 10.1016/j.cub.2018.05.054. Epub 2018 Jul 12.

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 3600
• Total vector size (bp): 7161
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: PTRE - tet activator responsive promoter
• Species: Synthetic

Gene/Insert 2

• Gene/Insert name: flex sequence
• Species: Synthetic

Gene/Insert 3

• Gene/Insert name: hM3dq-mCherry (inverted)
• Tag / Fusion Protein:
  • hM3Dq-mCherry

Gene/Insert 4

• Gene/Insert name: flex sequence

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The hM3dq-mCherry fragment was a gift from Bryan L. Roth, Addgene plasmid #44361.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry