pGE-attBTT-loxP-DsRed (pTL780)
(Plasmid #115160)

• Purpose: Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.
• Depositing Lab: Tobias Langenhan
• Publication: Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 10.1534/g3.118.200565.

Backbone

• Vector backbone: pGE
• Backbone size w/o insert (bp): 2986
• Total vector size (bp): 4284
• Vector type: Insect Expression, Cre/Lox
• Selectable markers: DsRed

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: attB-TT loxP 3xP3-DsRed
• Species: Synthetic
• Insert Size (bp): 1298

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: 5'-TCGAATTGCCGGCTGTACACTTA-3'
• 3′ sequencing primer: 5'-ACGAAGCGCCTCTATTTATA-3'

Resource Information

• Supplemental Documents:
  • pTL780.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on pGE-attB vector from Yang Hong (Huang et al., PNAS, 2009; DOI: 10.1073/pnas.0900641106)