pET32a-TRXtag
(Plasmid #11516)

• Depositing Lab: Pamela Bjorkman
• Publication: Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9.

Backbone

• Vector backbone: pET-32a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None - thioredoxin is vector encoded
• Alt name: trxA
• Alt name: TRX
• Alt name: E. coli thioredoxin
• Mutation: vector modified; removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.
• GenBank ID: AAN83133
• Entrez Gene: trxA
• Tags / Fusion Proteins:
  • Trx (N terminal on backbone)
  • His (C terminal on insert)
  • His (out of frame) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Novagen (vector derived)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry