pHD-mW-attPCC-FRT (pTL717)
(Plasmid #115158)

• Purpose: Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.
• Depositing Lab: Tobias Langenhan
• Publication: Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 10.1534/g3.118.200565.

Backbone

• Vector backbone: pHD-DsRed-attP
• Backbone size w/o insert (bp): 2796
• Total vector size (bp): 5974
• Vector type: Insect Expression
• Selectable markers: mini-White

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mW attP-CC FRT
• Species: Synthetic
• Insert Size (bp): 3178

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: 5'-CGTAGCGCCGATGGTAGTGTG-3' / 5'-CTCGCATATCTGGCTCTAAGACTTC-3'
• 3′ sequencing primer: 5'-CTACGCCCCCAACTGAGA-3' / 5'-ATGTTATCAGTATTTATTATGCA-3'

Resource Information

• Supplemental Documents:
  • pTL717

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on pHD-DsRed-attP vector (Gratz et al., Genetics, 2014; DOI: 10.1534/genetics.113.160713)