pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)
(Plasmid #115138)

• Purpose: CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS
• Depositing Labs: Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
• Publication: Marino et al Science. 2018 Sep 6. pii: science.aau5174. doi: 10.1126/science.aau5174.

Backbone

• Vector backbone: JDS246 (Plasmid #43861)
• Backbone manufacturer: Joung lab (Addgene Plasmid #43861)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human codon optimized AcrVA2 anti-CRISPR protein
• Alt name: AAS2283
• Species: Synthetic
• Tag / Fusion Protein:
  • NLS(SV40) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry