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Addgene

pReadthroughUGA
(Plasmid #11513)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRL-TK
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4045
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Firefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see comments (unknown if destroyed)
  • 3′ cloning site see comments (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The stop codon of the Firefly luciferase ORF from pGL3 was replaced with the stop codon UGA. This construct contains the Firefely ORF fused in frame to Renilla with 18 bp distance between the stop codon and the first ATG within the Renilla ORF. In addition, six CXCR4 short RNA binding sites are present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pReadthroughUGA was a gift from Phil Sharp (Addgene plasmid # 11513 ; http://n2t.net/addgene:11513 ; RRID:Addgene_11513)

  • For your References section:

    Short RNAs Repress Translation after Initiation in Mammalian Cells. Petersen CP, Bordeleau ME, Pelletier J, Sharp PA. Mol Cell. 2006 Feb 17. 21(4):533-42. 10.1016/j.molcel.2006.01.031 PubMed 16483934
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