pET32a-HD25Q
(Plasmid #11508)

• Depositing Lab: Pamela Bjorkman
• Publication: Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9.

Backbone

• Vector backbone: pET-32a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: huntingtin, exon 1, 25 glutamines
• Alt name: huntingtin (Huntington disease)
• Species: H. sapiens (human)
• Insert Size (bp): 298
• Mutation: huntingtin exon 1 with 25 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.
• GenBank ID: NM_002111
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Tags / Fusion Proteins:
  • Trx (N terminal on backbone)
  • His (C terminal on insert)
  • His (out of frame) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A. Kazantsev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry