pGEM-human TCR zeta/2470
(Plasmid #11507)

• Depositing Lab: Allan Weissman
• Publication: Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(24):9709-13.

Backbone

• Vector backbone: pGEM-3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3200
• Vector type: general cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCR zeta
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Mutation: n/a
• GenBank ID: J04132
• Entrez Gene: CD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry