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Addgene

pBabe neo human Frataxin
(Plasmid #11503)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE neo
  • Backbone manufacturer
    pBABE-neo is available at Addgene
  • Backbone size w/o insert (bp) 5330
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Frataxin
  • Alt name
    FRDA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    650
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was cloned before there was any consensus sequence for frataxin. There are 2 mutations with the currently available frataxin sequence - L140P R165C. The effect of these mutations is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe neo human Frataxin was a gift from Ronald Kahn (Addgene plasmid # 11503 ; http://n2t.net/addgene:11503 ; RRID:Addgene_11503)

  • For your References section:

    Frataxin activates mitochondrial energy conversion and oxidative phosphorylation. Ristow M, Pfister MF, Yee AJ, Schubert M, Michael L, Zhang CY, Ueki K, Michael MD, Lowell BB, Kahn CR. Proc Natl Acad Sci U S A. 2000 Oct 24. 97(22):12239-43. 10.1073/pnas.220403797 PubMed 11035806
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