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pCI-Neo-mdm2H452A/2858
(Plasmid #11497)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mdm2
  • Alt name
    hdm2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    H452A
  • GenBank ID
    NM_002392
  • Entrez Gene
    MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    AJ Levine, Princeton Univ PMID 8529093

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-mdm2H452A/2858 was a gift from Allan Weissman (Addgene plasmid # 11497 ; http://n2t.net/addgene:11497 ; RRID:Addgene_11497)

  • For your References section:

    Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53. Fang S, Jensen JP, Ludwig RL, Vousden KH, Weissman AM. J Biol Chem. 2000 Mar 24. 275(12):8945-51. 10.1074/jbc.275.12.8945 PubMed 10722742
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