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pGEX-mdm2C438L/2857
(Plasmid #11494)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Backbone manufacturer
    GE-Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mdm2
  • Alt name
    hdm2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    C438L
  • GenBank ID
    NM_002392
  • Entrez Gene
    MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer Amersham 5' pGEX
  • 3′ sequencing primer Amersham 3' pGEX
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    AJ Levine, Princeton Univ PMID 8529093

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-mdm2C438L/2857 was a gift from Allan Weissman (Addgene plasmid # 11494 ; http://n2t.net/addgene:11494 ; RRID:Addgene_11494)

  • For your References section:

    Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53. Fang S, Jensen JP, Ludwig RL, Vousden KH, Weissman AM. J Biol Chem. 2000 Mar 24. 275(12):8945-51. 10.1074/jbc.275.12.8945 PubMed 10722742
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