pGEX-mdm2C464A/2855
(Plasmid #11493)

• Depositing Lab: Allan Weissman
• Publication: Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-51.

Backbone

• Vector backbone: pGEX4T1
• Backbone manufacturer: GE-Amersham
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mdm2
• Alt name: hdm2
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: C464A
• GenBank ID: NM_002392
• Entrez Gene: MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: Amersham 5' pGEX
• 3′ sequencing primer: Amersham 3' pGEX

Resource Information

• A portion of this plasmid was derived from a plasmid made by: AJ Levine, Princeton Univ PMID 8529093
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry