pGEX-KG-MmUbc7/2783
(Plasmid
#11490)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonePGEX-KG
-
Backbone manufacturersee PMID 1852137
- Backbone size w/o insert (bp) 5000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMmUbc7
-
Alt nameUbe2g2
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)500
-
Mutationn/a
-
GenBank IDAF296657 NP_003334
-
Entrez GeneUbe2g2 (a.k.a. 1110003O05Rik, D10Xrf369, UBC7, Ubc7p)
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR I (not destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer Amersham 5' pGEX
- 3′ sequencing primer Amersham 3' pGEX (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX-KG-MmUbc7/2783 was a gift from Allan Weissman (Addgene plasmid # 11490 ; http://n2t.net/addgene:11490 ; RRID:Addgene_11490) -
For your References section:
Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s). Tiwari S, Weissman AM. J Biol Chem. 2001 May 11. 276(19):16193-200. 10.1074/jbc.M007640200 PubMed 11278356
