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pCI-Neo-HA-MmUbc6/2774
(Plasmid #11482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MmUbc6
  • Alt name
    Ube2j2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    780
  • Mutation
    n/a
  • GenBank ID
    AF296656 NP_919296
  • Entrez Gene
    Ube2j2 (a.k.a. Ubc6, Ubc6p, NCUBE-2, AL022923, 1200007B18Rik, 2400008G19Rik, 5730472G04Rik)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site Sac I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-HA-MmUbc6/2774 was a gift from Allan Weissman (Addgene plasmid # 11482 ; http://n2t.net/addgene:11482 ; RRID:Addgene_11482)

  • For your References section:

    Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s). Tiwari S, Weissman AM. J Biol Chem. 2001 May 11. 276(19):16193-200. 10.1074/jbc.M007640200 PubMed 11278356
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