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pcdna3mciap1 mut
(Plasmid #11476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mciap1mut
  • Alt name
    mciap1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Mutation
    582 to alanine
  • Entrez Gene
    Birc2 (a.k.a. A, AW146227, Api1, Api2, Birc3, C-IAP1, C330006D17Rik, HI, HIAP1, HIAP2, I, IAP1, IAP2, MI, MIAP1, MIAP2, MIH, MIHB, MIHC, RNF48, cI, cIA, cIAP1, cIAP2, mcI, mcIAP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII, (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3mciap1 mut was a gift from Jon Ashwell (Addgene plasmid # 11476 ; http://n2t.net/addgene:11476 ; RRID:Addgene_11476)

  • For your References section:

    Posttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Conze DB, Albert L, Ferrick DA, Goeddel DV, Yeh WC, Mak T, Ashwell JD. Mol Cell Biol. 2005 Apr . 25(8):3348-56. 10.1128/MCB.25.8.3348-3356.2005 PubMed 15798218
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