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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbcH5b
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Alt nameUbe2d2
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SpeciesH. sapiens (human)
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Insert Size (bp)444
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Mutationn/a
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GenBank IDU39317 NP_003330
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Entrez GeneUBE2D2 (a.k.a. E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nco I (not destroyed)
- 3′ cloning site BamH I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-15b-UbcH5b/2419 was a gift from Allan Weissman (Addgene plasmid # 11475 ; http://n2t.net/addgene:11475 ; RRID:Addgene_11475) -
For your References section:
Identification of a family of closely related human ubiquitin conjugating enzymes. Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM. J Biol Chem. 1995 Dec 22. 270(51):30408-14. 10.1074/jbc.270.51.30408 PubMed 8530467