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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemciap1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1700
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Entrez GeneBirc2 (a.k.a. A, AW146227, Api1, Api2, Birc3, C-IAP1, C330006D17Rik, HI, HIAP1, HIAP2, I, IAP1, IAP2, MI, MIAP1, MIAP2, MIH, MIHB, MIHC, RNF48, cI, cIA, cIAP1, cIAP2, mcI, mcIAP1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII, (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcdna3mciap1 was a gift from Jon Ashwell (Addgene plasmid # 11474 ; http://n2t.net/addgene:11474 ; RRID:Addgene_11474) -
For your References section:
Posttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Conze DB, Albert L, Ferrick DA, Goeddel DV, Yeh WC, Mak T, Ashwell JD. Mol Cell Biol. 2005 Apr . 25(8):3348-56. 10.1128/MCB.25.8.3348-3356.2005 PubMed 15798218