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pUC19_T790M_28bp
(Plasmid #114721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2658
  • Total vector size (bp) 2686
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    28
  • Mutation
    c.2369C>T (Substitution, position 2369, C➞T); p.Threonine 790 to Methionine
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MlyI (not destroyed)
  • 3′ cloning site ZraI (not destroyed)
  • 5′ sequencing primer TTTCCCAGTCACGACGTT
  • 3′ sequencing primer tgtggaattgtgagcgga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fragment contains 6 bp barcode TAGGAC at 3' end

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_T790M_28bp was a gift from Nils Walter (Addgene plasmid # 114721 ; http://n2t.net/addgene:114721 ; RRID:Addgene_114721)

  • For your References section:

    Ultra-specific and Amplification-free Quantification of Mutant DNA by Single-molecule Kinetic Fingerprinting. Hayward SL, Lund PE, Kang Q, Johnson-Buck A, Tewari M, Walter NG. J Am Chem Soc. 2018 Aug 20. doi: 10.1021/jacs.8b06685. 10.1021/jacs.8b06685 PubMed 30125495
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