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pET 15b-UbcH5a/2417
(Plasmid #11472)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11472 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UbcH5a
  • Alt name
    Ube2d1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • Mutation
    n/a
  • GenBank ID
    X78140 NP_00329
  • Entrez Gene
    UBE2D1 (a.k.a. E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    originally described by P.M. Howley PMID 8090726
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET 15b-UbcH5a/2417 was a gift from Allan Weissman (Addgene plasmid # 11472 ; http://n2t.net/addgene:11472 ; RRID:Addgene_11472)

  • For your References section:

    Identification of a family of closely related human ubiquitin conjugating enzymes. Jensen JP, Bates PW, Yang M, Vierstra RD, Weissman AM. J Biol Chem. 1995 Dec 22. 270(51):30408-14. 10.1074/jbc.270.51.30408 PubMed 8530467
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