pcDNA3-HA-AO7/2537
(Plasmid
#11470)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAO7
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Alt nameRNF25
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SpeciesM. musculus (mouse)
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Insert Size (bp)1500
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Mutationn/a
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GenBank IDAF171060
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Entrez GeneRnf25 (a.k.a. 0610009H16Rik, AO7)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR I (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH reverse primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-AO7/2537 was a gift from Allan Weissman (Addgene plasmid # 11470 ; http://n2t.net/addgene:11470 ; RRID:Addgene_11470) -
For your References section:
RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM. Proc Natl Acad Sci U S A. 1999 Sep 28. 96(20):11364-9. 10.1073/pnas.96.20.11364 PubMed 10500182