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Addgene

pRC02
(Plasmid #114681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114681 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETa
  • Backbone size w/o insert (bp) 2890
  • Total vector size (bp) 3901
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sfGFP.Strand10-Haloenzyme
  • Alt name
    His10-sfGFP10-Halo
  • Species
    Synthetic
  • Insert Size (bp)
    1011
  • Promoter T7
  • Tag / Fusion Protein
    • His10 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC02 was a gift from Matthew Good (Addgene plasmid # 114681 ; http://n2t.net/addgene:114681 ; RRID:Addgene_114681)

  • For your References section:

    Optochemical Control of Protein Localization and Activity within Cell-like Compartments. Caldwell RM, Bermudez JG, Thai D, Aonbangkhen C, Schuster BS, Courtney T, Deiters A, Hammer DA, Chenoweth DM, Good MC. Biochemistry. 2018 May 8;57(18):2590-2596. doi: 10.1021/acs.biochem.8b00131. Epub 2018 Apr 19. 10.1021/acs.biochem.8b00131 PubMed 29671583
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