pAL3
(Plasmid #114673)

• Purpose: (Empty Backbone) T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
• Depositing Lab: William Studier
• Publication: Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e63. doi: 10.1002/cpmb.63. Epub 2018 Jul 17.

Backbone

• Vector backbone: mosaic, has pBR322 ori
• Backbone size (bp): 5000
• Vector type: Bacterial Expression
• Promoter: T7
• Selectable markers: ccdB counter-selection
• Tag / Fusion Protein:
  • optional Ser-Gly-6His (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pAL3.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry